Primer

Part:BBa_K5103006:Design

Designed by: Nikoo Mansourian   Group: iGEM24_Guelph   (2024-09-22)


Forward primer introducing a HindIII cut site to the 5' end of Cry8Da


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We made sure there are no other HindIII cut sites within Cry8Da or pYES2 and PRS-316-PGK plasmids in order to cut them all with HindIII and successfully clone Cry8Da into them.


Figure 1. Forward primer that introduces HindIII cut site into Cry8Da.

Source

Part was created using SnapGene.

References